SOD2 Mediates Curcumin-Induced Protection against Oxygen-Glucose Deprivation/Reoxygenation Injury in HT22 Cells

Figure 2

Curcumin decreased cell injury in HT22 cells exposed to OGD/R and upregulated SOD2 expression in normal condition. The HT22 cells were divided into 5 groups, including control, OGD/R, and 3 concentrations (10 ng/ml, 100 ng/ml, and 500 ng/ml) of curcumin plus OGD/R groups. After the treatments, cell viability and LDH release were measured by using the MTT method and reagent kit, respectively. Then, the cells were divided into 4 groups, including control and 3 concentrations (10 ng/ml, 100 ng/ml, and 500 ng/ml) of curcumin treatment groups; after 3 h exposure, western blot was performed to assess SOD2 expression. (a) Curcumin restored cell viability (n = 8). (b) Curcumin reduced LDH release (n = 8). (c) Curcumin increased SOD2 expression (n = 4). Results are expressed as means ± SD. ; NS: no significance.

(a)

(b)

(c)