Jieduquyuziyin Prescription-Treated Rat Serum Suppresses Activation of Peritoneal Macrophages in MRL/Lpr Lupus Mice by Inhibiting IRAK1 Signaling Pathway

Figure 5

JP inhibits the activation of peritoneal macrophages in MRL/lpr lupus mice by downregulating IRAK1 and NF-κB signaling pathway. (a) After transfection and JP treatment, the expression alterations in IRAK1 and NF-κB mRNA. (b) First of all, JP could inhibit the expression of IRAK1 protein. (c) Next, after the JP intervention, the level of TRAF6 protein was inhibited. (d) Protein bands of each group of TRAF6. (e) Finally, the expression alterations in IKBα, p-IKBα, IKKα + IKKβ, NF-κB, and p-NF-κB protein after transfection and JP treatment. (f) Protein bands of each group of the related antibodies. Values are expressed as mean ± SD of three independent experiments. and versus vehicle control cells; and versus shRNA-treated cells.

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