The Tissue Distribution of Four Major Coumarins after Oral Administration of Angelicae Pubescentis Radix Extract to Rats Using Ultra-High-Performance Liquid Chromatography

Table 1

The precision and stabilities of columbianetin acetate, columbianetin, osthole, and columbianadin.

	Concentration; (ng/mL)	Intra-day precision		Inter-day precision		Autosampler for 24 hours stability
		Accuracy(%)	RSD(%)	Accuracy(%)	RSD(%)	Accuracy(%)	RSD(%)
Columbianetin	80	95.9	5.20	94.2	8.71	99.8	7.83
	500	93.9	0.35	96.2	2.11	97.9	2.89
	2000	93.8	0.74	97.3	1.91	96.0	9.48
Columbianetin acetate	80	93.6	3.05	93.5	7.32	97.4	2.55
	400	92.6	3.04	95.6	0.85	96.7	7.63
	2000	91.8	5.71	90.4	1.89	85.4	3.14
Osthole	160	97.3	5.29	98.3	4.32	99.2	5.28
	500	89.2	0.91	91.6	1.67	97.1	12.8
	2000	91.9	2.68	93.6	2.64	88.4	5.39
Columbianadin	160	92.6	5.77	93.8	6.54	97.1	5.73
	500	93.4	0.51	95.8	2.32	99.1	3.30
	2000	93.7	0.74	96.9	1.47	95.6	8.74