A Validated HPLC-MS/MS Method for Simultaneous Determination of Militarine and Its Three Metabolites in Rat Plasma: Application to a Pharmacokinetic Study

Figure 2

The chromatograms of the analytes and puerarin (IS) in rat plasma. (a) A blank rat plasma sample; (b) a blank rat plasma sample spiked with gastrodin (378.88 ng/mL), -isobutylmalic acid (209.92 ng/mL), gymnoside I (19.2 ng/mL), militarine (32.64 ng/mL) and IS (4 ng/mL); (c) a plasma sample at 30 min after oral administration militarine to rats. (1) Gastrodin, (2) -isobutylmalic acid, (3) gymnoside I, (4) militarine, and (5) puerarin (IS).

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(b)

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