A Validated HPLC-MS/MS Method for Simultaneous Determination of Militarine and Its Three Metabolites in Rat Plasma: Application to a Pharmacokinetic Study

Table 1

Mass spectra properties of the analytes and IS.

Analytes	Precursor ion (m/z)	Product ion (m/z)	Declustering potential (DP, V)	Entrance potential (EP, V)	Collision energy (CE, V)	Cell exit potential (CXP, V)
gastrodin	304.3	107.2	57	8	23	18
-isobutylmalic acid	189.0	129.0	-73	-10	-23	-13
gymnoside I	457.1	127.0	-77	-5	-32	-6
militarine	725.3	457.3	-43	-9	-22	-40
puerarin (IS)	417.1	267.1	110	7	35	6