A Validated HPLC-MS/MS Method for Simultaneous Determination of Militarine and Its Three Metabolites in Rat Plasma: Application to a Pharmacokinetic Study

Table 3

Summary of precision and accuracy of the four analytes in rat plasma (n=5).

Analytes	Spiked Concentration (ng/mL)	Intra-day			Inter-day
		Calculated concentration (ng/mL)	Precision (RSD, %)	Accuracy (%)	Calculated concentration (ng/mL)	Precision (RSD, %)	Accuracy (%)
militarine	2.04	2.22 ± 0.15	6.9	108.9	1.87 ± 0.21	11.4	91.5
	32.64	31.56 ± 2.37	7.5	96.7	36.73 ± 1.87	5.1	112.5
	97.92	105.10 ± 4.67	4.4	107.3	87.36 ± 5.97	6.8	89.2
gastrodin	5.92	6.48 ± 0.69	10.6	109.5	6.51 ± 0.63	9.7	109.9
	378.88	341.90 ± 18.12	5.3	90.2	335.42 ± 25.02	7.5	88.5
	1136.64	1253.71 ± 44.13	3.5	110.3	1051.16 ± 128.45	12.2	92.5
α-isobutylmalic acid	3.28	3.05 ± 0.38	12.5	93.0	3.59 ± 0.37	10.3	109.4
	209.92	230.72 ± 13.11	5.7	109.9	217.31 ± 27.23	12.5	103.5
	629.76	672.08 ± 49.73	7.4	106.7	575.98 ± 51.20	8.9	91.5
gymnoside I	0.6	0.58 ± 0.05	8.3	96.6	0.52 ± 0.05	10.1	87.4
	19.2	16.99 ± 1.97	11.6	88.5	17.53 ± 2.32	13.2	91.3
	57.6	52.70 ± 2.82	5.4	91.5	61.33 ± 5.70	9.3	106.5