A Validated HPLC-MS/MS Method for Simultaneous Determination of Militarine and Its Three Metabolites in Rat Plasma: Application to a Pharmacokinetic Study

Table 4

Summary of recovery and matrix effect of the four analytes in rat plasma (n=5).

Analytes	Spiked Concentration (ng/mL)	Extraction Recovery		Matrix effect
		Mean ± SD (%)	RSD (%)	Mean ± SD (%)	RSD (%)
militarine	2.04	105.34 ± 7.91	7.5	102.82 ± 8.53	8.3
	32.64	92.40 ± 10.65	11.5	87.61 ± 11.49	13.1
	97.92	89.92 ± 8.99	10.0	92.52 ± 4.75	5.1
gastrodin	5.92	85.71 ± 6.01	7.0	88.80 ± 9.22	10.4
	378.88	92.49 ± 5.40	5.8	85.43 ± 4.63	5.4
	1136.64	87.58 ± 8.20	9.4	92.76 ± 6.78	7.3
α-isobutylmalic acid	3.28	93.14 ± 3.56	3.8	93.48 ± 8.07	8.6
	209.92	89.10 ± 10.88	12.2	84.42 ± 10.91	12.9
	629.76	95.71 ± 10.17	10.6	89.30 ± 10.13	11.3
gymnoside I	0.6	93.02 ± 3.53	3.8	85.41 ± 3.42	4.0
	19.2	90.40 ± 12.08	13.4	90.14 ± 8.92	9.9
	57.6	83.52 ± 2.86	3.4	88.30 ± 10.78	12.2