Traditional Herbal Formula Taeeumjowi-Tang (TJ001) Inhibits p53-Mutant Prostate Cancer Cells Growth by Activating AMPK-Dependent Pathway

Figure 4

TJ001 had no effects on apoptosis in DU145 cells. (a) Cells (1X10²) were grown with or without TJ001 (200 μg/mL) in 60pi dish for 48 h. Doxorubicin (DOX) was also used as a positive control. Harvested cells were stained with annexin-V-FITC for detecting apoptosis, then were stained with 7-AAD for detecting DNA contents for 15 min each. Cell apoptosis was determined by FACS analysis. (b) Apoptosis-regulated protein levels were evaluated after TJ001 (200 μg/mL) treatment. Anti-β-actin was a loading control.

(a)

(b)