Protium javanicum Burm. Methanol Extract Attenuates LPS-Induced Inflammatory Activities in Macrophage-Like RAW264.7 Cells
Figure 1
Effects of Pj-ME on NO production in LPS-activated macrophages. ((a) and (d)) Murine macrophage-like RAW264.7 cells or peritoneal macrophages pretreated with Pj-ME (0-200 μg/ml) or L-NAME (0-1 mM) for 30 min and then treated with LPS (1 μg/ml) for 24 h. LPS-induced NO production levels were determined by the Griess assay. ((b) and (e)) To evaluate the cytotoxic activity of Pj-ME or L-NAME, RAW264.7, and HEK293 cells, and peritoneal macrophages were treated with Pj-ME (0-200 μg/ml) and L-NAME (0-1.5 mM) for 24 h. Cell viability was then determined by the MTT assay. (c) Phytochemical fingerprinting was performed by LC/MS spectrophotometric analysis. Putative components were included in each peak. Data ((a), (b), (d), and (e)) expressed as mean ± SD are representative of 3 independent experiments. ##: p< 0.01 with respect to the untreated group; p< 0.05 and p< 0.01 with respect to the LPS-treated group.