Protium javanicum Burm. Methanol Extract Attenuates LPS-Induced Inflammatory Activities in Macrophage-Like RAW264.7 Cells

Figure 4

Effect of Pj-ME on the upstream JAK/STAT3 signaling cascade in LPS-stimulated RAW264.7 cells. (a) Western blot analysis was performed to determine protein expression levels in whole cell lysates of RAW264.7 cells treated with Pj-ME (200 μg/ml) for 30 min followed by LPS treatment (1 μg/ml) over different amounts of time. Levels of phosphorylated and total STAT3 and JAK2 at 6, 9, 12, and 24 h were determined with their specific antibodies. β-Actin was used as a loading control. (b) STAT3-specific expression of IL-6 was determined by real-time PCR from LPS-treated RAW264.7 cells. Data (b) expressed as mean± SD are representative of 3 independent experiments. ^##p< 0.01 with respect to untreated group and p< 0.01 with respect to treated group.

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