Susceptibility of Toxoplasma gondii to Ethanolic Extract of Tinospora crispa in Vero Cells

Figure 1

Microscopic observation of intracellular parasite at 24 hours and 48 hours postinfection treatment. Treatment of infected cells with EETC for 24 hours (a) and 48 hours (d), treatment with clindamycin for 24 hours (b) and 48 hours (e), and negative control for 24 hours (c) and 48 hours (f). The arrow indicates the parasitophorous vacuole in Vero cells where the tachyzoites proliferate. It can be observed that the normal rosette arrangement of the intracellular tachyzoite as observed in the negative control (c and f) is lost in the EETC (a and d) and to some extent in clindamycin treated group (b and e) with some empty vacuoles or vacuoles with reduced intracellular tachyzoites. Magnification ×400, scale bar 20 μm.

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