Fermented Garlic Ameliorates Hypercholesterolemia and Inhibits Platelet Activation

Figure 4

Effect of fermented and nonfermented preparations of garlic on expression of SREBP-2, ACAT-1, ACAT-2, and HMG-CoA. The liver tissue from rats treated with various samples and fed a high cholesterol diet was excised, and RNA was extracted by homogenizing with TRIzol solution. cDNA was obtained using reverse transcriptase, and the resulting cDNA was subjected to PCR with the primers of SREBP-2, ACAT-2, and HMG-CoA. GAPDH was used as the housekeeping gene. The obtained product was electrophoresed, and gel images were obtained as shown in (a). Gel electrophoresis was performed in triplicate. The intensity of gel images was quantified using ImageJ software and normalized against the housekeeping gene GAPDH as shown in bar graph (a). (b) After reverse transcriptase, the resultant cDNA was then added with SYBR Green, with primers of GAPDH, ACAT-1, and ACAT-2 for real-time PCR analysis. compared with the control.

(a)

(b)