Preventive Effect of Small-Leaved Kuding Tea (Ligustrum robustum (Roxb.) Bl.) Polyphenols on D-Galactose-Induced Oxidative Stress and Aging in Mice

Table 2

Sequences of primers used in this study.

Gene Name	Sequence
nNOS	Forward: 5′-ACGGCAAACTGCACAAAGC-3′
	Reverse: 5′-CGTTCTCTGAATACGGGTTGTTG-3′
eNOS	Forward: 5′-TCAGCCATCACAGTGTTCCC-3′
	Reverse: 5′-ATAGCCCGCATAGCGTATCAG-3′
iNOS	Forward: 5′-GTTCTCAGCCCAACAATACAAGA-3′
	Reverse: 5′-GTGGACGGGTCGATGTCAC-3′
Cu/Zn-SOD	Forward: 5′-AACCAGTTGTGTTGTCAGGAC-3′
	Reverse: 5′-CCACCATGTTTCTTAGAGTGAGG-3′
Mn-SOD	Forward: 5′-CAGACCTGCCTTACGACTATGG-3′
	Reverse: 5′-CTCGGTGGCGTTGAGATTGTT-3′
CAT	Forward: 5′- GGAGGCGGGAACCCAATAG -3′
	Reverse: 5′- GTGTGCCATCTCGTCAGTGAA-3′
HO-1	Forward: 5′-ACAGATGGCGTCACTTCG-3′
	Reverse: 5′-TGAGGACCCACTGGAGGA-3′
Nrf2	Forward: 5′-CAGTGCTCCTATGCGTGAA-3′
	Reverse: 5′-GCGGCTTGAATGTTTGTC-3′
γ-GCS	Forward: 5′-GCACATCTACCACGCAGTCA-3′
	Reverse: 5′-CAGAGTCTCAAGAACATCGCC-3′
NQO1	Forward: 5′-CTTTAGGGTCGTCTTGGC-3′
	Reverse: 5′-CAATCAGGGCTCTTCTCG-3′
GAPDH	Forward: 5′-AGGTCGGTGTGAACGGATTTG-3′
	Reverse: 5′-GGGGTCGTTGATGGCAACA-3′

nNOS: neuronal nitric oxide synthase; eNOS: endothelial nitric oxide synthase; iNOS: inducible nitric oxide synthase; Cu/Zn-SOD: cuprozinc-superoxide dismutase; Mn-SOD: manganese superoxide dismutase; CAT: catalase; HO-1: heme oxygenase-1; Nrf2: nuclear factor-erythroid 2 related factor 2; γ-GCS: γ-glutamylcysteine synthetase; NQO1: NAD(P)H dehydrogenase [quinone] 1; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.