Lycopus lucidus Turcz Inhibits the Osteoclastogenesis in RAW 264.7 Cells and Bone Loss in Ovariectomized Rat Model

Figure 3

wLL inhibits the expression of TRAF-6 in RANKL-induced RAW 264.7 cells. RAW 264.7 cells were seeded at 2 × 10⁶/well on a 60 mm culture plate. After 24 hours, RAW 264.7 cells were incubated with RANKL (100 ng/ml) for indicated times with or without the presence of LL (100 μg/mL). The cells were lysed with RIPA buffer, and expression of TRAF-6 was evaluated with western blot. (A) Expression of TRAF-6. (B) Expression of TRAF-6 normalized to actin. Data represent the mean ± SEM of the three independent experiments.