Lycopus lucidus Turcz Inhibits the Osteoclastogenesis in RAW 264.7 Cells and Bone Loss in Ovariectomized Rat Model

Figure 4

wLL inhibits the expression of c-fos and NFATc-1 in RANKL-induced RAW 264.7 cells. RAW 264.7 cells were seeded at 5 × 10⁵/well on a 60 mm culture plate. After 24 hours, RAW 264.7 cells were incubated with RANKL (100 ng/ml) for 4 days with or without the presence of LL (1, 10, and 100 μg/mL). The cells were lysed with RIPA buffer; protein expressions of c-fos and NFATC-1 were evaluated with western blot. To analysis of mRNA expression, the cells were extracted using a TriZol kit, and mRNA expressions of c-fos and NFATc1 were determined by RT-PCR. (a) Protein and mRNA expression of c-fos and NFATc-1. (b) Protein expression of c-fos and NFATc1 normalized to actin; mRNA expression of c-fos and NFATc1 normalized to GAPDH. Data represent the mean ± SEM of the three independent experiments.

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