Lycopus lucidus Turcz Inhibits the Osteoclastogenesis in RAW 264.7 Cells and Bone Loss in Ovariectomized Rat Model

Table 1

Primers, sequences, and PCR conditions.

Primer name	Primer Sequence	Annealing Tm (°C)	cycle
TRAP	5′-ACTTCCCCAGCCCTTACTACCG-3′; 5′-TCAGCACATAGCCCACACCG-3′	58°C	32
NFATc1	5′-TGCTCCTCCTCCTGCTGCTC-3′; 5′-CGTCTTCCACCTCCACGTCG-3′	58°C	32
c-Fos	5′-ATG GGC TCT CCT GTC AAC AC-3′; 5′-GGCTGCCAA AATAAACTCCA-3′	58°C	33
RANK	5′-AAACCTTGGACCAACTGCAC-3′; 5′-ACCATCTTCTCCTCCCGAGT-3′	53°C	32
CAII	5′-CTCTCAGGACAATGCAGTGCTGA-3′; 5′-ATCCAGGTCACACATTCCAGCA-‘3	58°C	33
CTK	5′-AGGCGGCTATATGACCACTG-3′; 5′-CCGAGCCAAGAGAGCATATC-‘3	58°C	28
OSCAR	5′-CTGCTGGTAACGGATCAGCTCC CCAGA-3′; 5′-CCAAGGAGCCAGAACCTTCGAAACT-3′	53°C	40
ATP6v0d2	5′-ATGGGGCCTTGCAAAAGAAATCTG-3′; 5′-CGACAGCGTCAAACAAAGGCTTGTA-3	58°C	30
GAPDH	5′-ACTTTGTCAAGCTCATTTCC-3′; 5′-TGCAGCGAACTTTATTGATG-3′	57°C	30