Studies on Antibacterial Activity and Diversity of Cultivable Actinobacteria Isolated from Mangrove Soil in Futian and Maoweihai of China

Figure 5

Induction of a two-color dual reporter system sensitive to inhibitors of the ribosome progression or inhibitors of DNA replication, respectively. Spots of erythromycin (Ery), levofloxacin (Lev), and tested samples were placed on the surface of an agar plate containing E. coli tolC cells transformed with the pDualrep2 reporter plasmid. Shown is the fluorescence of the lawn of E. coli cells scanned at 553/574 nm (green pseudocolor) for RFP fluorescence and 588/633 nm (red pseudocolor) for Katushka2S fluorescence. Induction of Katushka2S expression is triggered by translation inhibitors, while RFP is upregulated by induction of DNA damage SOS response. 176: 10X7D-1-3; 177: 7X8A-5; 438: s1d5-4; 452: s7b4-1; 454: s1b9-3.