Effect of Propolis Preparations on Transepithelial Electrical Potential, Resistance, and Ion Transport in In Vitro Study

Figure 1

The electrical potential in stationary conditions before stimulation. The figure shows arrangement the electrical potential value before individual stimulation. The experiment were performed according to scheme stimulations by Ringer solution (RH), bumetanide (B), amiloride (AMI) and amiloride with bumetanide (AB). The time of each stimulation was 15 seconds. The figure shows also first and second simulation by Ringer solution (stimulation RH₁ and stimulation RH₂) and stimulation by bumetanide (stimulation B) stimulation by amiloride in bumetanide (stimulation AB). Abbreviations. p < 0.001, RH: Ringer fluid, stimulations 1 and 2, B: bumetanide solution (0.1 mM), AB: a mixture of amiloride and bumetanide solutions (0.1 mM). I: long-term incubation in 1 ml ethanol extract of propolis dissolved in 100 ml water, II: undisturbed ion transport, and III: long-term incubation in propolis ointment;