Bu Shen Zhu Yun Decoction Improves Endometrial Receptivity via VEGFR-2-Mediated Angiogenesis

Figure 4

BSZYD induced HEMEC angiogenesis via the MAPK signaling pathway. (a) After incubation in low-serum medium for 24 h, HEMECs were treated with BSZYD, followed by incubation with or without MAPK signaling pathway inhibitors for 2 h. The cells were treated with or without VEGF (40 ng/mL) for indicated times points. Phospho-ERK, phospho-JNK, and phospho-P38 levels were determined with western blotting using specific antibodies (left panel); densitometric scanning (right panel). Values are expressed as mean ± SD from three independent experiments. as compared with the control group; as compared with VEGF group. (b-d) MMP9, PCNA, and cyclin D1 levels were determined with western blotting (left panel). Densitometric scanning (right panel). Values are expressed as means ± SD from three independent experiments. as compared with the control group; as compared with the VEGF group.

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