Anti-Inflammatory Effects of Licania macrocarpa Cuatrec Methanol Extract Target Src- and TAK1-Mediated Pathways

Figure 1

Effects of Lm-ME on NO production and cell viability. (a) Macrophage-like RAW264.7 cells and primary macrophage cells obtained from the peritoneal exudates of thioglycollate- (TG-) injected mice were pretreated with 50, 100, or 200 μg/ml of Lm-ME for 30 min before treatment with LPS for 24 h NO production was measured using the cell culture supernatant. (b) RAW264.7 cells were pretreated with 0.5, 1, or 2 mM L-NAME and then treated with LPS. After 24 h incubation, NO collected from cell culture supernatants was measured by Griess solution. (c) RAW264.7 cells, HEK293T cells, and peritoneal macrophages were treated Lm-ME 50, 100, or 200 μg/ml. After 24 h, cell viability was measured by MTT assay. (d) Cytotoxicity of L-NAME was determined by MTT assay. (e, f) Components of Lm-ME were analyzed by HPLC.

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