Anti-Inflammatory Effects of Licania macrocarpa Cuatrec Methanol Extract Target Src- and TAK1-Mediated Pathways

Figure 3

Inhibitory effects of Lm-ME on the NF-κB and AP-1 pathways. (a) RAW264.7 cells were pretreated 200 μg/ml of Lm-ME for 30 min, followed by 1 μg/ml of LPS for the indicated times (5, 15, 30, or 60 min). Cell lysates were prepared and the phosphorylated and total forms of IKKα/β, p50, p65, and IκBα were determined by western blotting analysis. (b) Lm-ME-pretreated RAW264.7 cells were exposed to LPS for the indicated times (3 or 5 min), and cell lysates were obtained. Phosphorylated and total forms of Src and p85 were checked by western blotting analysis. (c–e) RAW 264.7 cells were pretreated with 200 μg/ml of Lm-ME and incubated with LPS for the indicated times (3, 5, 15, 30, or 60 min). Phosphorylated and total forms of ERK, JNK, p38, MEK1/2, MKK4, MKK3/6, and MKK7 or TAK1, IRAK4, and IRAK1 were determined by western blotting analysis.

(a)

(b)

(c)

(d)

(e)