Cytoprotective Effect of Ligustrum robustum Polyphenol Extract against Hydrogen Peroxide-Induced Oxidative Stress via Nrf2 Signaling Pathway in Caco-2 Cells

Table 1

Primers used for the amplification of Nrf2 and related genes.


Gene	Primer Sequences	Fragment	Annealing	Accession Number

Nrf2	F: AGT GGA TCT GCC AAC TAC TC	105bp	60°C	NM001313904.1
	R: CAT CTA CAA ACG GGA ATG TCT G
NQO1	F: CGC AGA CCT TGT GAT ATT CCA GT	87bp	60°C	NM001025433.1
	R: TCC TAT GAA CAC TCG CTC AAA CC
HO-1	F: CCA GGC AGA GAA TGC TGA GT	156bp	58°C	NM002133.2
	R: GTA GAC AGG GGC GAA GAC TG
GCL	F: ATG GAG GTG CAA TTA ACA GAC	204bp	58°C	XM017010749.1
	R: CTG CAT TGC CAC CTT TGC A
β-actin	F: GGC ACC CAG CAC AAT GAA	160bp	58°C	NM001101.3
	R: CTA AGT CAT AGT CCG CCT AGA AGC A