Evidence-Based Complementary and Alternative Medicine / 2019 / Article / Fig 2

Research Article

Protective Effects of Aqueous Extract of Mentha suaveolens against Oxidative Stress-Induced Damages in Human Keratinocyte HaCaT Cells

Figure 2

Effect of MSAE on ROS generation in HaCaT keratinocyte cells. Cells were pretreated with different concentrations of MSAE for 18 h or 5 μM sulforaphane followed by treatment with 1.6 mM H2O2 for 30 min. These cells were incubated at 37°C in the dark for 30 min with culture medium containing 25 μM DCF-DA. Intracellular ROS accumulation was detected by ELISA (a) and fluorescence microscopy. (b) Data are expressed as meanSD of three independent experiments. , compared with untreated cells; , ; , compared with H2O2-treated cells.

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