Safety Assessment of Methanol Extract of Melastoma malabathricum L. Leaves following the Subacute and Subchronic Oral Consumptions in Rats and Its Cytotoxic Effect against the HT29 Cancer Cell Line
Table 3
Haematological profiles of Sprague-Dawley rats following the 28-day oral pretreatment with vehicle (Tween 80) or MEMM in the subacute toxicity study.
Haematological parameters
Male
Female
Control
Treatment
Control
Treatment
Tween 80
MEMM (mg/kg)
Tween 80
MEMM (mg/kg)
—
500
1000
—
500
1000
RBC (×1012/L)
8.36 ± 0.22
8.42 ± 0.31
8.66 ± 0.77
7.73 ± 0.93
7.89 ± 0.83
9.46 ± 0.47
WBC (×109/L)
3.74 ± 0.41
3.02 ± 0.81
4.86 ± 0.93
4.83 ± 0.51
6.30 ± 1.62
3.62 ± 0.70
Hb (g/L)
138.40 ± 3.06
129.40 ± 5.04
122.20 ± 8.41
125.71 ± 11.44
108.40 ± 13.11
134.00 ± 12.36
PCV (L/L)
0.36 ± 0.01
0.33 ± 0.01
0.31 ± 0.02
0.41 ± 0.10
0.27 ± 0.03
0.32 ± 0.02
MCV (fL)
43.40 ± 2.51
39.00 ± 1.05
35.80 ± 2.65
37.21 ± 0.62
34.60 ± 0.87
34.20 ± 1.99
MCHC (g/L)
382.80 ± 7.81
395.20 ± 6.49
401.20 ± 10.85
388.67 ± 22.21
393.80 ± 18.00
413.20 ± 16.46
Neutrophils (×109/L)
10.80 ± 1.20
10.60 ± 1.94
9.50 ± 2.5
12.61 ± 1.27
14.20 ± 2.60
16.00 ± 2.52
Lymphocytes (×109/L)
71.60 ± 1.60
79.20 ± 3.40∗
81.25 ± 3.84
68.77 ± 5.34
57.00 ± 4.67
73.33 ± 2.73
Monocytes (×109/L)
5.00 ± 0.55
6.40 ± 1.44
5.08 ± 0.41
5.41 ± 0.52
6.40 ± 1.03
4.67 ± 0.33
Eosinophils (×109/L)
3.40 ± 0.75
2.40 ± 0.24
2.75 ± 0.48
3.22 ± 0.43
4.20 ± 1.72
4.00 ± 1.16
Values are expressed as means ± SEM. Groups (n = 6): control (treated with Tween 80); treatments (treated with 500 mg/kg MEMM and 1000 mg/kg MEMM).
