Safety Assessment of Methanol Extract of Melastoma malabathricum L. Leaves following the Subacute and Subchronic Oral Consumptions in Rats and Its Cytotoxic Effect against the HT29 Cancer Cell Line
Table 4
Haematological profiles of Sprague-Dawley rats following the 90-day oral pretreatment with vehicle (Tween 80) or MEMM in the subchronic toxicity study.
Haematological parameters
Male
Control
Treatment
Tween 80
MEMM (mg/kg)
—
50
500
1000
RBC (×1012/L)
8.15 ± 0.32
8.25 ± 0.17
6.98 ± 0.91
6.61 ± 0.73
WBC (×109/L)
153.30 ± 9.42
155.80 ± 2.50
133.31 ± 15.80
132.80 ± 14.73
Hb (g/L)
0.40 ± 0.02
0.41 ± 0.01 0
0.34 ± 0.04
0.35 ± 0.04
PCV (L/L)
49.25 ± 0.75
49.75 ± 1.32
49.25 ± 1.60
52.25 ± 0.63
MCV (fL)
383.00 ± 11.71
380.80 ± 10.04
393.00 ± 6.55
385.30 ± 6.30
MCHC (g/L)
8.44 ± 1.86
7.85 ± 0.69
11.08 ± 3.12
8.30 ± 1.87
Neutrophils (×109/L)
2.51 ± 0.50
3.48 ± 0.58
3.03 ± 0.92
3.53 ± 0.83
Lymphocytes (×109/L)
4.89 ± 1.16
3.60 ± 0.20
6.42 ± 1.24
3.92 ± 0.93
Monocytes (×109/L)
0.55 ± 0.14
0.46 ± 0.08
0.61 ± 0.14
0.53 ± 0.17
Eosinophils (×109/L)
0.32 ± 0.08
0.32 ± 0.05
0.35 ± 0.04
0.32 ± 0.02
Values a reexpressed as means ± SEM. Groups (n = 6): control (treated with Tween 80); treatments (treated with 50 mg/kg MEMM, 250 mg/kg MEMM, and 1000 mg/kg MEMM)
