Safety Assessment of Methanol Extract of Melastoma malabathricum L. Leaves following the Subacute and Subchronic Oral Consumptions in Rats and Its Cytotoxic Effect against the HT29 Cancer Cell Line
Table 5
Biochemical profiles of liver of Sprague-Dawley rats following the 28-day oral pretreatment with vehicle (Tween 80) or MEMM in the subacute toxicity study.
Serum liver parameters
Male
Female
Control
Treatment
Control
Treatment
Tween 80
MEMM (mg/kg)
Tween 80
MEMM (mg/kg)
—
500
1000
—
500
1000
ALT (U/L)
83.44 ± 21.06
107.80 ± 33.25
138.10 ± 33.18
90.71 ± 10.18
240.60 ± 77.36
94.18 ± 31.23
ALP (U/L)
154.80 ± 23.57
153.80 ± 22.98
98.60 ± 9.42
122.26 ± 14.35
103.80 ± 8.02
115.80 ± 12.48
AST (U/L)
352.40 ± 78.28
342.30 ± 65.90
227.73 ± 55.90
381.77 ± 48.37
332.04 ± 36.80
344.71 ± 38.70
TBil (μmol/L)
0.80 ± 0.08
0.62 ± 0.09
0.76 ± 0.04
0.41 ± 0.07
0.28 ± 0.07
0.24 ± 0.09
Creatinine (μmol/L)
33.60 ± 1.36
41.40 ± 8.79
38.40 ± 6.56
36.57 ± 8.21
47.00 ± 8.01
32.40 ± 5.79
Urea (mmol/L)
8.64 ± 0.58
10.73 ± 0.67
10.83 ± 0.60
9.52 ± 0.94
11.95 ± 0.19
6.97 ± 0.87
Values are expressed as means ± SEM. Groups (n = 6): control (treated with Tween 80); treatments (treated with 500 mg/kg MEMM and 1000 mg/kg MEMM).