Reproductive Regulation and Oxidative Stress Alleviation of Chinese Herbal Medicine Therapy in Ovariectomised Mouse Model
Chromatographic fingerprint analysis and cytotoxicity assay of the Chinese herbal formula B401. (a) Chromatographic fingerprint analysis was conducted through the LC/MS. Fifteen bioactive marker substances from the components of the herbal formula B401 were qualitatively determined within 80 min under selected LC/MS conditions. (b) (A) Cell viability of herbal formula B401 treatment measured through the MTT assay of SH-SY5Y cells. (B) The IC50 values of the Chinese herbal formula B401 in SH-SY5Y cells were calculated using the dose–response curve. The results are shown as mean ± SEM, and experiments were repeated three times for each treatment.