Chromatographic fingerprint analysis and cytotoxicity assay of the Chinese herbal formula B401. (a) Chromatographic fingerprint analysis was conducted through the LC/MS. Fifteen bioactive marker substances from the components of the herbal formula B401 were qualitatively determined within 80 min under selected LC/MS conditions. (b) (A) Cell viability of herbal formula B401 treatment measured through the MTT assay of SH-SY5Y cells. (B) The IC50 values of the Chinese herbal formula B401 in SH-SY5Y cells were calculated using the dose–response curve. The results are shown as mean ± SEM, and experiments were repeated three times for each treatment.