Danzhi Jiangtang Capsule Mediates NIT-1 Insulinoma Cell Proliferation and Apoptosis by GLP-1/Akt Signaling Pathway

Figure 1

The cellular proliferation in NIT-1 cells. NIT-1 cells were cultured with rats’ serums containing high dose or low dose of DJC for 24h; then, they were additionally treated with 110⁻⁵ mmol⋅L⁻¹ GLP-1 receptor inhibition or 5umol⋅L⁻¹ AKT inhibition for another 24h. (a) The cell viability assay using CCK-8 kit; (b) Q-PCR analysis of PDX-1 mRNA level changes. Data were shown as Mean ± SD. Each group was independently repeated for three times. CTL, control; HG, high glucose; LD, low dose of DJC; HD, high dose of DJC; GI, GLP-1 receptor inhibition; AI, AKT inhibition. <0.05, <0.01, <0.001.

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