Qingshen Buyang Formula Attenuates Renal Fibrosis in 5/6 Nephrectomized Rats via Inhibiting EMT and Wnt/β-Catenin Pathway

Figure 4

Qingshen Buyang Formula inhibited activated Wnt/β-catenin signaling pathway. (a) Representative western blot analyses illustrated that Qingshen Buyang Formula suppressed the protein expression of Wnt4, TCF4, and p-GSK3β involved in Wnt/β-catenin signaling pathway, while the expression of GSK3β had no change. ((b) and (c)) Graphic presentation of Wnt4, TCF4, and p-GSK3β/GSK3β in the three groups as indicated. ((d)–(f)) Real-time PCR revealed that Qingshen Buyang Formula reversed the increased mRNA expression of Wnt4, TCF4, and β-catenin. P< 0.05, compared with the Sham group. # P< 0.05, compared with the 5/6Nx group. The error bars represent the standard deviation of measurements for 8 particles in three separate sample runs (n = 24).

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