FuFangChangTai Decoction Activates Macrophages via Inducing Autophagy

Figure 1

FFCT induces autophagy in colon cancer: (a) effect of FFCT on the viability of CT26.WT cells. P < 0.05 versus the control group; (b) formation of intracellular autophagy marker eGFP-LC3 II on CT26.WT cells by fluorescence microscope. A. Negative control, B. CT26.WT cells were treated with FFCT (10 mg/mL, 48 h), C. FFCT (20 mg/mL, 48 h), D. FFCT (30 mg/mL, 48 h), E. FFCT (40 mg/mL, 48 h), F. positive control, CT26.WT cells were treated with rapamycin (50 nM, 48 h); (c) effect of FFCT on the expression of LC3 protein in CT26.WT cells after 48 h by western blot assay.

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