Evidence-Based Complementary and Alternative Medicine / 2019 / Article / Fig 5

Research Article

Pueraria lobata Extract Protects Hydrogen Peroxide-Induced Human Retinal Pigment Epithelial Cells Death and Membrane Permeability

Figure 5

Effect of P. lobata on ZO-1 expression in RPE cells. Cells were pretreated with P. lobata extract (10 μg/mL) and its single compounds (1 μM) for 60 min and then cultured for 24 h in the presence of H2O2. (a) Western blotting analysis of ZO-1 expression. (b) Immunohistochemistry for ZO-1 in H2O2-induced cells treated with P. lobata extract. Immunofluorescence of RPE monolayers shows the beneficial effect of P. lobata (10 μg/mL) and its single compounds (1 μM) in RPE cells against H2O2-induced tight junction expression. ZO-1 (a–c, red), DAPI (a’–c’, blue), and merged images (a”–c”).

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