Brazilian Green Propolis Inhibits Ox-LDL-Stimulated Oxidative Stress in Human Umbilical Vein Endothelial Cells Partly through PI3K/Akt/mTOR-Mediated Nrf2/HO-1 Pathway

Figure 1

Effects of ethanol extracted Brazilian green propolis (EEBP) on cell viability in Ox-LDL treated HUVECs. (a), Effects of Ox-LDL (20, 40, and 80 μg/mL) on cell viability in Ox-LDL induced HUVECs at 6 h. (b), Effects of EEBP (1.25, 2.5, and 5 μg/mL) on cell viability in Ox-LDL induced HUVECs for 3, 6, and 12 h. Cell viability was tested by SRB assay and illustrated in column figures. (P < 0.05, P < 0.01 vs Ox-LDL, n=3). Data are means ± S.E.M. (c), AO staining showed treatment with EEBP depressed nuclear condensation and fragmentation in Ox-LDL induced HUVECs (200×). (d), Hoechst 33258 staining suggested that treatment with EEBP inhibited apoptosis in Ox-LDL induced HUVECs (200×).

(a)

(b)

(c)

(d)