Evaluation of the Biological Effects of Lyophilized Hydrophilic Extract of Rhus coriaria on Myeloperoxidase (MPO) Activity, Wound Healing, and Microbial Infections of Skin Wound Tissues

Table 5

Effect of aqueous extract of Rhus coriaria fruits on infected wounds.

Treatment	Wounds infected with Gram positive (Staph. aureus)
	Wound area (cm^2) on day			Scar area (mm^2)	Period of epithelization (Days)
	3	6	9
G5	1.6 ± 0.06	1.4 ± 0.06	1.1 ± 0.06	91.8± 1.4	19.5± 0.6
G6	1.54 ± 0.04	1.16 ± 0.03	0.75 ± 0.05	97.3± 3.5	16.0± 0.0
AERc
G7 (5 mg.mL^−1)	1.4± 0.12	0.58 ± 0.08	0.08 ± 0.03	98.7± 1.9	12.0± 0.0
G8 (10 mg.mL^−1)	1.2± 0.11	0.35 ± 0.02	0.06 ± 0.01	99.8± 0.4	10.8± 0.1
Treatment	Wounds infected with Gram Negative (P. aeruginosa)
	Wound area (cm^2) on day			Scar area (mm^2)	Period of epithelization (Days)
	3	6	9
AERc
G9 (5 mg.mL^−1)	1.5± 0.19	1.2 ± 0.11	0.87 ± 0.21	94.7± 2.9	13.0± 0.0
G10 ( 10 mg.mL^−1)	1.4± 0.18	0.95 ± 0.12	0.75 ± 0.10	99.1± 0.4	12.0± 0.0

Values represent the mean ± SD (n=10). Statistical differences were determined by ANOVA followed Student-Newman-Keuls test. G5: infected rats with either Staphylococcus aureus (ATCC 25923) or Pseudomonas aeruginosa received topical application (200 μL) of solutions containing saline (NaCl, 0.9%); G6: infected rats with either Staphylococcus aureus (ATCC 25923) or Pseudomonas aeruginosa received 1% silver sulfadiazine (Dermazine®) topically as standard treatment once daily; G7: infected rats with Staphylococcus aureus (ATCC 25923) treated with AERc extract at doses of 5 mg.kg-1 for once a day for 9 consecutive days; G8: infected rats with Staphylococcus aureus (ATCC 25923) treated with AERc extract at doses of 10 mg.kg-1 for once a day for 9 consecutive days; G9: infected rats with Pseudomonas aeruginosa received AERc extract at doses of 5 mg.kg-1 for once a day for 9 consecutive days; G10: infected rats with Pseudomonas aeruginosa received AERc extract at doses of 10 mg.kg-1 for once a day for 9 consecutive days. P<0.001, P<0.01 as compared to respective saline treatment. P<0.001 as compared to Dermazine-treated animals.