Dracohodin Perochlorate Stimulates Fibroblast Proliferation via EGFR Activation and Downstream ERK/CREB and PI3K/Akt/mTOR Pathways In Vitro

Figure 2

DP promotes fibroblast proliferation. Cells in 96-well plates were cultured with different concentrations of DP for 24 h. (a) Cell viability was measured by CCK-8 assay, and the results showed that DP (>0.5 μg/mL) effectively promoted cell proliferation, with a peak at 3 μg/mL. (b) Fibroblasts were treated by DP (0, 2, 3, and 4 μg/mL) for 0, 6, 12, 24, and 36 h, respectively. We determined that DP stimulated cell proliferation in a time-dependent manner (data are presented as mean values ± SD, n = 15 for each bar; ; , ^## and ^●● vs. control group at the same time).

(a)

(b)