Dracohodin Perochlorate Stimulates Fibroblast Proliferation via EGFR Activation and Downstream ERK/CREB and PI3K/Akt/mTOR Pathways In Vitro

Figure 6

Inhibitors suppressed DP-induced fibroblast cell proliferation. (a) The effects of inhibitors on fibroblast proliferation were detected using CCK-8. (b) In subsequent experiments, the experimental groups were divided into DP-treated group without inhibitor pretreatment (DP group), DP + AG1478, DP + U0126, DP + LY294002, DP + U0126 + LY294002, DP + ICG001, DP + BEZ235, and DP + ICG001 + BEZ235. The cells without DP treatment were used as the control group. Cells were pretreated with inhibitors individually for 12 h and then were cultured in a complete medium containing 3 μg/mL DP for 24 h. Finally, the cell viability was assessed using CCK-8. The results showed that DP-stimulated cell proliferation was inhibited by related inhibitors (data are presented as means ± SD, n = 15 for each bar, vs. control group, ^## vs. DP-treated group without inhibitor pretreatment).

(a)

(b)