Preferential alteration of the tubulin cytoskeleton in the SKOV-3 ovarian cancer cells by XFC. Human ovarian clear cell carcinoma (ES-2) and human ovarian adenocarcinoma (SKOV-3) cells were cultured as described in Section 2
. Treatment with various concentrations of XFC or with nocodazole was performed in serum-free media for 24 hours. Immunostaining of tubulin was performed with antitubulin antibody. DAPI was used as a nuclear stain. Fluorescence microscopy was used for data acquisition in (a) ES-2 and (b) SKOV-3 ovarian cancer cells. (c) Total fluorescence was acquired and the data was processed as described in Section 2.