XFC extract inhibits CDK4 and cyclin D3 in SKOV-3 ovarian cancer cells. Human ovarian clear cell carcinoma (ES-2) and human ovarian adenocarcinoma (SKOV-3) cells were cultured as described in Section 2
. Treatment with various concentrations of XFC was performed in serum-free media for 24 hours. Cell lysates were harvested and then processed for (a) SDS-PAGE and western blotting in order to assess the expression levels of Cdk2, CDk4, Cdk6, Cyclin D1, Cyclin D3, p27, and GAPDH. (b) Levels of expression were quantified using scanning densitometry.