Aconiti Lateralis Radix Preparata, the Dried Root of Aconitum carmichaelii Debx., Improves Benign Prostatic Hyperplasia via Suppressing 5-Alpha Reductase and Inducing Prostate Cell Apoptosis

Table 1

Analysis conditions for LC-MS.

LC-MS condition
Component	Agilent 1290 Infinity II UHPLC
	G6650A Q-TOF MS (Agilent)
Ion source	Dual AJS ESI
Polarity (kV)	Positive mode (4.0)
	Negative mode (3.5)
Mass range (m/z)	20–1700
Reference masses (m/z)	Positive mode with 121.0509 and 922.0098
	Negative mode with 112.9856 and 966.0007
LC column	Agilent Eclipse Plus C18 RRHD column (50 mm × 2.1 mm, 1.8 μm)
LC flow rate	0.300 mL/min
Injection volume	1.00 μl
Column temperature	30°C
Solvent composition	A: 0.1% formic acid in water
	B: 0.1% formic acid in ACN
Mobile phase	Time (min)	A (%)	B (%)
	0.0	95	5
	3.0	95	5
	13.0	10	90
	15.0	10	90
	17.0	95	5
	20.0	95	5