Chinese Medicine FTZ Recipe Protects against High-Glucose-Induced Beta Cell Injury through Alleviating Oxidative Stress

Figure 4

Effects of FTZ serum on high-glucose (25 mM)-induced cytotoxicity in INS-1 cells. INS-1 cells were treated with different concentrations of glucose and different concentrations of FTZ serum for 24 h. Cell viability was assessed using the CCK-8 assay kit. Cell viability was measured to calculate the percentage of untreated INS-1 cells (a). Apoptosis rate by flow cytometry with Annexin V-FITC/PI double staining (b, c) after INS-1 cells were treated with control (11 mM glucose +vehicle); (B) Model (25 mM glucose +vehicle); (C) 200 μM edaravone, (D) 10% rat serum, (E) 0.4% FTZ serum, (F) 2. 0% FTZ serum, and (G) 10% FTZ serum for 24 h before incubation with 25 mM glucose for 24 h. n=6 in each group. Compared with the control group, ^#P<0.05; ^##P < 0.01. Compared with model group, P<0.05; P<0.01.

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