Chinese Medicine FTZ Recipe Protects against High-Glucose-Induced Beta Cell Injury through Alleviating Oxidative Stress

Figure 6

Effect of FTZ serum on the level of ROS in INS-1 cells under 25 mM glucose-induced oxidative stress. Cells were untreated or pretreated with different concentrations of FTZ serum or edaravone for 24 h before incubation with 25mM glucose (HG) for 24 h. (A) Fluorescence (DCF)-labeled cells in (a) untreated control; (b) HG⁺ + 0.1% DMSO; (c) HG⁺ + 30 μM edaravone; (d) HG+ 10% rat serum; (e) HG+ 0.4% FTZ serum; (f) HG+ 2% FTZ serum; and (g) HG⁺ + 10% FTZ serum were observed under a microscope with a scale bar of 100 μm. (B) The ROS fluorescence intensity (Intracellular ROS generation) in each group was calculated by the rate of untreated naïve INS-1 cells. Values represent the mean ± SD in 3 independent experiments. Compared with the control group, ^##P < 0.01. Compared with model group, P<0.05; P<0.01.