Effect of Rhus verniciflua Extract on IgE-Antigen-Mediated Allergic Reaction in Rat Basophilic Leukemic RBL-2H3 Mast Cells and Passive Cutaneous Anaphylaxis in Mice

Figure 5

Effect of Rhus verniciflua extract (RVE) on the Ag/IgE-induced passive cutaneous anaphylaxis (PCA) model. ICR mice were subcutaneously injected with DNP-IgE (4 μg/mL) into the ear. After 24 h, mice were orally administered with RVE (250 or 500 mg/mL) and dexamethasone (10 mg/mL). After 1 h, DNP–HSA (300 μg/mL) containing 1% Evans blue was intravenously injected into their tail veins for 1 h The extravasated dye in the ears was analyzed using the procedure described in the Materials and Methods section. Results are expressed as mean ± SE of at least five independent experimental results that were tested by analysis of variance with Bonferroni’s post hoc testing; versus the control group; versus the DNP-Ag-treated group.