Development of a HPLC-MS/MS Method to Determine the 13 Elements of Semen Cuscutae and Application to a Pharmacokinetic Study in Rats

Table 3

Precision and accuracy of 13 analytes in rat plasma (n = 6).

Compounds	Spiked concentration (ng/mL)	Intraday			Interday
		Measured concentration (ng mL^−1)	Accuracy (RE, %)	Precision (RSD, %)	Measured concentration (ng mL^−1)	Accuracy (RE, %)	Precision (RSD, %)
Quercitrin	2	1.94 ± 0.05	−3.0	2.6	1.95 ± 0.05	−2.5	2.6
	25	27.65 ± 1.75	10.6	6.3	26.16 ± 0.94	4.6	3.6
	250	253.47 ± 4.06	1.4	1.6	264.45 ± 10.46	5.8	4.0
	2	1.99 ± 0.10	−0.5	5.0	2.02 ± 0.04	1.0	2.0
Quercetin	25	26.60 ± 1.02	6.4	3.8	25.56 ± 0.68	2.2	2.7
	250	262.38 ± 5.97	5.0	2.3	252.55 ± 3.26	1.0	1.3
	2	1.98 ± 0.12	−1.0	6.1	2.05 ± 0.05	2.5	2.4
Hyperoside	25	28.58 ± 0.33	14.3	1.2	28.21 ± 0.39	12.8	1.4
	250	277.08 ± 12.08	10.8	4.4	270.47 ± 4.99	8.2	1.8
Caffeic acid	2	2.14 ± 0.06	7.0	2.8	2.00 ± 0.06	−0.1	3.0
	25	25.92 ± 1.78	3.7	6.9	25.83 ± 0.47	3.3	1.8
	250	257.37 ± 5.90	2.9	2.3	250.56 ± 2.29	0.2	0.9
Rutin	4	4.24 ± 0.17	6.0	4.0	4.16 ± 0.13	4.0	3.1
	50	55.09 ± 2.26	10.2	4.1	49.67 ± 1.34	−0.7	2.7
	500	494.24 ± 8.21	−1.2	1.7	498.04 ± 5.47	−0.4	1.1
Chlorogenic acid	2	1.98 ± 0.03	−1.0	1.5	1.90 ± 0.15	−5.0	7.9
	25	26.10 ± 2.72	4.4	10.4	26.28 ± 0.73	5.1	2.8
	250	254.48 ± 7.30	1.8	2.9	254.24 ± 4.74	1.7	1.9
	2	2.18 ± 0.14	9.0	6.4	1.96 ± 0.05	−2.0	2.6
Luteolin	25	26.98 ± 1.74	7.9	6.4	25.97 ± 1.16	3.9	4.5
	250	256.22 ± 6.42	2.5	2.5	255.18 ± 3.16	2.1	1.2
Apigenin	2	2.12 ± 0.14	6.0	6.6	2.03 ± 0.09	1.5	4.4
	25	27.22 ± 1.31	8.9	4.8	25.60 ± 0.48	2.4	1.9
	250	258.13 ± 10.48	3.3	4.1	261.87 ± 13.20	4.7	5.0
Kaempferol	2	2.03 ± 0.06	1.5	3.0	2.05 ± 0.10	2.5	4.9
	25	25.92 ± 0.84	3.7	3.2	25.43 ± 0.58	1.7	2.3
	250	253.01 ± 7.68	1.2	3.0	266.49 ± 14.27	6.6	5.4
Isoquercitrin	2	2.02 ± 0.07	1.0	3.5	2.00 ± 0.07	0.1	3.5
	25	28.62 ± 0.62	14.5	2.2	28.29 ± 1.06	13.2	3.7
	250	272.50 ± 4.96	9.0	1.8	273.47 ± 8.62	9.4	3.2
Cryptochlorogenic acid	2	2.03 ± 0.06	1.5	3.0	2.00 ± 0.08	0.1	4.0
	25	25.92 ± 0.84	3.7	3.2	25.61 ± 1.03	2.4	4.0
	250	253.01 ± 7.68	1.2	3.0	251.30 ± 1.99	0.5	0.8
Isorhamnetin-3-O-glucoside	2	1.99 ± 0.07	−0.5	3.5	2.02 ± 0.05	1.0	2.5
	25	26.18 ± 0.85	4.7	3.2	25.53 ± 0.58	2.1	2.3
	250	254.51 ± 1.60	1.8	0.6	250.00 ± 3.23	0.0	1.3
Astragalin	2	1.95 ± 0.05	−2.5	2.6	2.00 ± 0.04	−0.1	2.0
	25	26.19 ± 0.93	4.8	3.6	25.07 ± 0.39	0.3	1.6
	250	248.93 ± 1.24	−0.4	0.5	253.07 ± 2.55	1.2	1.0