Development of a HPLC-MS/MS Method to Determine the 13 Elements of Semen Cuscutae and Application to a Pharmacokinetic Study in Rats

Table 4

Extraction recoveries and matrix effects of the analytes (n = 6).

Compounds	Concentration (ng/mL)	Extraction recovery (%)	RSD (%)	Matrix effect (%)	RSD (%)
Quercitrin	2	97.3 ± 5.1	5.2	105.8 ± 4.8	4.5
	25	97.5 ± 2.8	2.9	102.1 ± 2.4	2.4
	250	87.7 ± 2.0	2.3	106.7 ± 4.3	4.0
Quercetin	2	89.9 ± 7.3	8.1	115.5 ± 11.6	10.0
	25	98.7 ± 3.8	3.9	93.5 ± 7.2	7.7
	250	87.7 ± 1.8	2.1	97.0 ± 4.3	4.4
Hyperoside	2	103.9 ± 4.4	4.2	101.4 ± 6.2	6.1
	25	102.3 ± 6.3	6.2	97.1 ± 5.7	5.9
	250	101.7 ± 0.6	0.6	81.1 ± 2.9	3.6
Caffeic acid	2	98.8 ± 7.1	7.2	101.0 ± 4.5	4.5
	25	102.0 ± 2.9	2.8	86.9 ± 11.2	12.9
	250	96.2 ± 4.8	5.0	87.4 ± 3.0	3.4
Rutin	4	103.2 ± 4.6	4.5	103.3 ± 3.9	3.8
	50	92.0 ± 3.7	4.0	95.3 ± 7.2	7.6
	500	90.7 ± 5.2	5.7	85.9 ± 10.7	12.5
Chlorogenic acid	2	103.6 ± 5.1	4.9	97.9 ± 9.1	9.3
	25	97.8 ± 2.0	2.0	98.2 ± 6.1	6.2
	250	95.4 ± 1.3	1.4	85.1 ± 6.4	7.5
Luteolin	2	106.2 ± 4.5	4.2	95.3 ± 6.0	6.3
	25	95.3 ± 3.5	3.7	103.9 ± 9.9	9.5
	250	87.4 ± 2.6	3.0	97.4 ± 2.4	2.5
Apigenin	2	99.1 ± 6.7	6.8	95.4 ± 4.5	4.7
	25	91.4 ± 2.6	2.8	81.0 ± 2.3	2.8
	250	88.5 ± 1.5	1.7	88.7 ± 2.1	2.4
Kaempferol	2	99.1 ± 5.2	5.2	105.6 ± 15.2	14.4
	25	94.7 ± 4.2	4.4	96.6 ± 3.8	3.9
	250	91.4 ± 1.7	1.9	85.9 ± 1.0	1.2
Isoquercitrin	2	104.3 ± 2.2	2.1	97.3 ± 2.7	2.8
	25	104.7 ± 6.7	6.4	93.9 ± 2.3	2.4
	250	101.8 ± 2.6	2.6	83.2 ± 2.1	2.5
Cryptochlorogenic acid	2	94.7 ± 4.9	5.2	94.5 ± 7.6	8.0
	25	94.0 ± 3.6	3.8	111.2 ± 1.7	1.5
	250	90.8 ± 3.1	3.4	81.9 ± 3.1	3.8
Isorhamnetin-3-O-glucoside	2	99.3 ± 5.4	5.4	109.9 ± 3.8	3.5
	25	96.4 ± 5.2	5.4	99.8 ± 5.0	5.0
	250	88.8 ± 2.5	2.8	102.3 ± 3.4	3.3
Astragalin	2	97.9 ± 5.1	5.2	115.1 ± 4.9	4.3
	25	98.7 ± 3.9	4.0	98.7 ± 1.2	1.2
	250	87.6 ± 3.9	4.5	103.8 ± 4.9	4.7