Research Article
Development of a HPLC-MS/MS Method to Determine the 13 Elements of Semen Cuscutae and Application to a Pharmacokinetic Study in Rats
Table 5
Stability of all analytes in rat plasma (n = 6).
| Compounds | Spiked concentration (ng/mL) | Room temperature for 4h | Three freeze-thaw | Autosampler for 12h | −70°C for 21 days | Measured (ng/mL) | RSD (%) | Measured (ng/mL) | RSD (%) | Measured (ng/mL) | RSD (%) | Measured (ng/mL) | RSD (%) |
| Quercitrin | 2 | 2.1 ± 0.1 | 4.8 | 2.3 ± 0.1 | 4.3 | 2.1 ± 0.1 | 4.8 | 2.0 ± 0.1 | 5.0 | 25 | 26.3 ± 0.7 | 2.7 | 26.9 ± 1.5 | 5.6 | 26.4 ± 0.9 | 3.4 | 26.5 ± 0.5 | 1.9 | 250 | 252.5 ± 9.1 | 3.6 | 261.4 ± 10.3 | 3.9 | 253.1 ± 4.7 | 1.9 | 247.9 ± 5.3 | 2.1 | Quercetin | 2 | 2.2 ± 0.1 | 4.5 | 2.0 ± 0.1 | 5.0 | 2.3 ± 0.1 | 4.3 | 1.9 ± 0.1 | 5.3 | 25 | 26.2 ± 0.7 | 2.7 | 27.3 ± 0.6 | 2.2 | 26.6 ± 0.7 | 2.6 | 25.6 ± 0.9 | 3.5 | 250 | 254.9 ± 11.8 | 4.6 | 253.2 ± 5.0 | 2.0 | 245.3 ± 11.6 | 4.7 | 260.0 ± 6.2 | 2.4 | Hyperoside | 2 | 2.0 ± 0.1 | 5.0 | 2.1 ± 0.1 | 4.8 | 1.8 ± 0.1 | 5.6 | 1.9 ± 0.1 | 5.3 | 25 | 23.0 ± 1.2 | 5.2 | 26.2 ± 1.5 | 5.7 | 23.6 ± 0.7 | 3.0 | 26.4 ± 1.8 | 6.8 | 250 | 244.4 ± 14.3 | 5.9 | 254.7 ± 9.7 | 3.8 | 230.2 ± 13.1 | 5.7 | 256.4 ± 15.8 | 6.2 | Caffeic acid | 2 | 2.1 ± 0.1 | 4.8 | 2.1 ± 0.1 | 4.8 | 2.2 ± 0.1 | 4.5 | 2.0 ± 0.1 | 5.0 | 25 | 27.9 ± 1.0 | 3.6 | 27.0 ± 1.3 | 4.8 | 28.1 ± 0.8 | 2.8 | 26.0 ± 0.6 | 2.3 | 250 | 272.5 ± 8.9 | 3.3 | 255.7 ± 3.9 | 1.5 | 262.6 ± 7.4 | 2.8 | 250.2 ± 5.1 | 2.0 | Rutin | 4 | 3.9 ± 0.2 | 5.1 | 4.0 ± 0.1 | 2.5 | 4.0 ± 0.2 | 5.0 | 4.0 ± 0.1 | 2.5 | 50 | 56.3 ± 2.3 | 4.1 | 57.7 ± 1.0 | 1.7 | 49.8 ± 2.7 | 5.4 | 52.7 ± 3.9 | 7.4 | 500 | 505.7 ± 13.0 | 2.6 | 513.1 ± 7.4 | 1.4 | 489.3 ± 14.2 | 2.9 | 494.4 ± 8.8 | 1.8 | Chlorogenic acid | 2 | 2.1 ± 0.1 | 4.8 | 2.1 ± 0.1 | 4.8 | 2.1 ± 0.1 | 4.8 | 2.1 ± 0.1 | 4.8 | 25 | 27.7 ± 1.6 | 5.8 | 28.1 ± 1.0 | 3.6 | 26.6 ± 1.6 | 6.0 | 26.6 ± 1.2 | 4.5 | 250 | 258.4 ± 15.7 | 6.1 | 268.6 ± 15.8 | 5.9 | 253.1 ± 6.0 | 2.4 | 253.6 ± 7.7 | 3.0 | Luteolin | 2 | 2.2 ± 0.1 | 4.5 | 2.0 ± 0.1 | 5.0 | 2.1 ± 0.1 | 4.8 | 2.0 ± 0.1 | 5.0 | 25 | 26.5 ± 0.5 | 1.9 | 26.8 ± 1.6 | 6.0 | 25.6 ± 1.0 | 3.9 | 27.4 ± 0.9 | 3.3 | 250 | 256.7 ± 11.5 | 4.5 | 261.1 ± 8.2 | 3.1 | 244.9 ± 6.3 | 2.6 | 257.6 ± 5.1 | 2.0 | Apigenin | 2 | 2.2 ± 0.1 | 4.5 | 2.0 ± 0.1 | 5.0 | 2.1 ± 0.2 | 9.5 | 2.1 ± 0.1 | 4.8 | 25 | 26.4 ± 1.2 | 4.5 | 25.7 ± 2.0 | 7.8 | 25.0 ± 1.2 | 4.8 | 26.5 ± 1.3 | 4.9 | 250 | 249.3 ± 12.8 | 5.1 | 262.2 ± 16.0 | 6.1 | 248.5 ± 4.9 | 2.0 | 257.8 ± 8.2 | 3.2 | Kaempferol | 2 | 2.2 ± 0.2 | 9.1 | 2.0 ± 0.2 | 10.0 | 2.1 ± 0.2 | 9.5 | 2.0 ± 0.1 | 5.0 | 25 | 24.8 ± 0.3 | 1.2 | 26.2 ± 1.6 | 6.1 | 25.4 ± 2.5 | 9.8 | 25.7 ± 0.8 | 3.1 | 250 | 247.5 ± 6.3 | 2.5 | 252.6 ± 7.5 | 3.0 | 254.0 ± 4.9 | 1.9 | 257.2 ± 6.4 | 2.5 | Isoquercitrin | 2 | 1.8 ± 0.2 | 11.1 | 2.1 ± 0.1 | 4.8 | 1.9 ± 0.1 | 5.3 | 1.9 ± 0.1 | 5.3 | 25 | 22.8 ± 1.4 | 6.1 | 26.2 ± 1.8 | 6.9 | 24.0 ± 0.5 | 2.1 | 25.9 ± 1.8 | 6.9 | 250 | 247.2 ± 14.7 | 5.9 | 248.9 ± 5.1 | 2.0 | 238.3 ± 7.2 | 3.0 | 263.6 ± 10.2 | 3.9 | Cryptochlorogenic acid | 2 | 2.0 ± 0.1 | 5.0 | 2.0 ± 0.1 | 5.0 | 2.1 ± 0.1 | 4.8 | 2.0 ± 0.1 | 5.0 | 25 | 27.0 ± 1.8 | 6.7 | 26.2 ± 0.3 | 1.1 | 27.3 ± 0.6 | 2.2 | 25.5 ± 1.2 | 4.7 | 250 | 260.4 ± 3.3 | 1.3 | 248.9 ± 6.5 | 2.6 | 251.2 ± 9.7 | 3.9 | 244.4 ± 7.4 | 3.0 | Isorhamnetin-3-O-glucoside | 2 | 2.0 ± 0.1 | 5.0 | 2.0 ± 0.1 | 5.0 | 2.1 ± 0.1 | 4.8 | 2.1 ± 0.1 | 4.8 | 25 | 27.1 ± 1.3 | 4.8 | 27.1 ± 1.5 | 5.5 | 26.9 ± 1.0 | 3.7 | 26.2 ± 0.6 | 2.3 | 250 | 259.9 ± 5.5 | 2.1 | 255.0 ± 5.7 | 2.2 | 251.5 ± 5.4 | 2.1 | 249.5 ± 7.0 | 2.8 | Astragalin | 2 | 2.1 ± 0.1 | 4.8 | 2.1 ± 0.1 | 4.8 | 2.1 ± 0.1 | 4.8 | 2.0 ± 0.1 | 5.0 | 25 | 27.6 ± 1.2 | 4.3 | 27.2 ± 0.8 | 2.9 | 27.3 ± 1.0 | 3.7 | 26.1 ± 1.2 | 4.6 | 250 | 248.1 ± 4.3 | 1.7 | 258.6 ± 8.5 | 3.3 | 254.6 ± 4.6 | 1.8 | 251.5 ± 8.7 | 3.5 |
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