Safflower Yellow B Protects Brain against Cerebral Ischemia Reperfusion Injury through AMPK/NF-kB Pathway

Figure 4

SYB pretreatment protected PC12 cells from I/R-induced cytotoxicity and apoptosis (a). Protective effects of SYB on I/R-induced cytotoxicity in PC12 cells. PC12 cells viability was assessed by measuring the MTT reduction. Results were shown as fold of control (b). Effects of SYB on the LDH leakage induced by I/R (c). Effects of SYB on PC12 cells apoptosis induced by I/R. PC12 Cells were subjected to I/R with or without SYB pretreatment, then double stained with Annexin V/propidium iodide (PI) (d). The expression levels of cleaved-caspase 3, Bax, and Bcl-2 were measured by Western blotting. Data were presented as mean ± SD. ^##P<0.01 versus control group; .01 versus I/R group.

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