Effect of Puerarin, Baicalin and Berberine Hydrochloride on the Regulation of IPEC-J2 Cells Infected with Enterotoxigenic Escherichia coli

Figure 6

Rate of apoptosis in IPEC-J2 cells. (a) Apoptosis was detected in IPEC-J2 cells by flow cytometry. The normal rate of apoptosis in IPEC-J2 cells was 4.64%. ETEC infection for 3 h significantly increased apoptosis to 7.67%. (b) Pretreatment with baicalin and berberine hydrochloride did not significantly decrease the apoptosis. Control: untreated cells; ETEC: treatment with ETEC for 3 h; puerarin: pretreatment with puerarin 200 μg/mL + ETEC; baicalin: pretreatment with baicalin 1 μg/mL + ETEC; berberine: pretreatment with berberine hydrochloride 100 μg/mL + ETEC. Data are mean ± SEM, n = 3 per treatment (P < 0.05; P < 0.01).

(a)

(b)