Effect of Puerarin, Baicalin and Berberine Hydrochloride on the Regulation of IPEC-J2 Cells Infected with Enterotoxigenic Escherichia coli

Figure 8

Confocal microscopy of IPEC-J2 cells and quantification of fluorescence intensities of NF-κB P65 in the nucleus. (a) Confocal microscopy of IPEC-J2 cells. The cells were stained for the nucleus (blue) and the protein NF-κB P65 (red). Top row: untreated IPEC-J2 cells; second row: treatment with ETEC for 3 h; third row: pretreatment with puerarin 200 μg/mL + ETEC; fourth row: pretreatment with baicalin 1 μg/mL + ETEC; bottom row: pretreatment with berberine hydrochloride 100 μg/mL + ETEC. Bar scale represents 10 μm. (b) Quantification of fluorescence intensities of NF-κB P65 in the nucleus. ETEC infection increased the fluorescence intensities of NF-κB P65 in the nucleus. Relative to the ETEC infection group alone, pretreatment with baicalin and berberine hydrochloride significantly decreased the fluorescence intensities of NF-κB P65 in the nucleus. Control: untreated cells; ETEC: treatment with ETEC for 3 h; puerarin: pretreatment with puerarin at 200 μg/mL + ETEC; baicalin: pretreatment with baicalin at 1 μg/mL + ETEC; berberine: pretreatment with berberine hydrochloride at 100 μg/mL + ETEC. Data are mean ± SEM, n = 6 per treatment (P < 0.05).

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