Brain Cortical and Hippocampal Dopamine: A New Mechanistic Approach for Eurycoma longifolia Well-Known Aphrodisiac Activity and Its Chemical Characterization

Table 1

Peak assignments of E. longifolia aqueous extract metabolites using UPLC-MS/MS in positive and negative ionization modes.

Peak	(min)	[M-H]^-	[M+H]^-	Molecular formula	Identification	MS^2
1	15.01		351.100		Eurycolactone B	352.000, 315.010, 290.800
2	20.05		365.000		6α-Hydroxyeurycomalactone	347.000, 318.900, 202.100
3	21.92		409.000		Eurycomanone	391.000, 373.200, 345.100, 225.000
4	23.63	411.100	413.200		13β,18-dihydroeurycomanol	395.100, 376.800, 319.000, 291.200
5	23.90	443.300			13β,21-dihydroxyeurycomanol	427.100, 413.000, 379.300
6	24.21	571.100	573.100		Eurycomanol-2-O-β-D-glucopyranoside	375.200, 357.000, 299.100
7	29.54		383.200		15β-Hydroxyklaineanone	365.100, 326.800, 266.000, 202.100
8	29.98	395.100			14,15β-Dihydroxyklaineanone	377.000, 359.100, 349.300
9	38.36		413.100		5α, 14β, 15β-Trihydroxyklaineanone	395.200
10	38.43	315.100			Laurycolactone B	271.200, 256.001
11	45.76		455.102		6α-Acetoxy-14,15β-dihydroxyklaineanone	454.100, 436.000
12	45.91	391.100			(α/β-epoxide) Ailanthone	373.100, 363.200
13	46.18	573.122			Iandonoside B	555.000, 513.900
14	46.52		251.200		9-Methoxycanthin-6-one	236.100, 233.000, 216.200
15	46. 69	279.000			5,9-Dimethoxycanthin-6-one	264.000, 251.100, 237.000
16	47.45	265.100			11-Hydroxy-10-methoxycanthin-6-one	250.200, 247.300, 222.200
17	47.91		389.000		2-Hydroxy-3,2′,6′-trimethoxy-4′-(2,3-epoxy-1-hydroxypropyl)-5-(3-hydroxy-1-propenyl)-biphenyl	371.110, 357.001, 343.100, 295.000