Enhancement of Endometrial Receptivity by Cnidium officinale through Expressing LIF and Integrins

Figure 2

The effect of CoM on cytotoxicity and the adhesion of JAr cell to Ishikawa cells. (a) Ishikawa cells (1.5 × 10⁶ cells) were seeded and incubated with CoM at indicated concentration (up to 500 μg/ml) for 24 h. The cell viability was measured by MTT assay. The data were expressed as percentage of controls and are shown as mean ± SEM of three independent experiments. (b) Ishikawa cells were treated with CoM (50 μg/mL) for 48 h. CMFDA-labeled JAr cells were added onto the CoM-treated Ishikawa cells. The attached JAr cells were fixed, pictured using fluorescent microscopy, and manually counted. Data were calculated as mean ± SEM of three independent experiments. The data were expressed as fold of controls. for comparison between two groups (magnification ×50; scale bar = 5 μm).

(a)

(b)