Enhancement of Endometrial Receptivity by Cnidium officinale through Expressing LIF and Integrins

Figure 5

In vivo effect of CoM administration on embryo implantation. To confirm improvement of embryo implantation by CoM, female mice were orally administered with CoM (67.75 mg/kg/day) in 100 μL of normal saline solution for 15 days. On day 4 from pregnancy estimated by vaginal plugs, the female mice were injected with RU486 (4 mg/kg/day) for 4 days. All mice were sacrificed, and both uterine horns were excised to examine the number of implanted embryos. The number of implantation sites was calculated as means ± SEM. Uterine tissue sections were analyzed histologically after hematoxylin and eosin staining. and for comparisons between two groups.